Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM
نویسندگان
چکیده
منابع مشابه
Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM.
TraM is essential for F plasmid-mediated bacterial conjugation, where it binds to the plasmid DNA near the origin of transfer, and recognizes a component of the transmembrane DNA transfer complex, TraD. Here we report the 1.40 A crystal structure of the TraM core tetramer (TraM58-127). TraM58-127 is a compact eight-helical bundle, in which the N-terminal helices from each protomer interact to f...
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The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers bridge the origin of transfer (oriT) to a key component of the conjugative pore, the coupling protein TraD. Here we show that TraM recognizes a high-affinity DNA-binding site, sbmA, as a cooperative dimer of tetramers. The cryst...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2006
ISSN: 0261-4189,1460-2075
DOI: 10.1038/sj.emboj.7601151